Use of chimeric adenoviral vectors to assess capsid neutralization determinants

Use of adenoviral vectors with a minimal cytomegalovirus promoter.

E1-deleted recombinant adenoviruses 5 are widely used as gene vectors for basic and clinical research. Most adenoviral constructions bear an expression cassette inserted into the E1 region of their genome close to the 5′ end and include a heterologous promoter. The major immediate early human cytomegalovirus (hCMV) and the Rous sarcoma virus promoters, which have fairly high and consistent acti...

Chimeric influenza haemagglutinins: Generation and use in pseudotype neutralization assays

Recently chimeric influenza haemagglutinins (cHAs) have been generated as potential 'universal' vaccination antigens and as tools to identify HA stalk-directed antibodies via their use as antigens in ELISA, and virus or pseudotype-based neutralization assays. The original methods [1], [2] used for their generation require the amplification of regions of interest (head and stalk) using primers c...

Construction of first-generation adenoviral vectors.

Genetically modified adenoviruses (Ads) make attractive vectors for the delivery of exogenous DNA to mammalian cells for basic science and gene therapy applications. Ad vector production consists of (1) cloning a trangene into an infectious plasmid by in vivo recombination in bacteria, (2) rescuing and propagating the vector in complementing cells, and (3) purifying the vector. All of this can ...

Strains of Feline Calicivirus Exchanges from Antigenically Distinct Containing Capsid Protein Domain Specificities of Chimeric Viruses Recovery and Altered Neutralization

Recovery and altered neutralization specificities of chimeric viruses containing capsid protein domain exchanges from antigenically distinct strains of feline calicivirus.

Feline calicivirus (FCV) strains can show significant antigenic variation when tested for cross-reactivity with antisera produced against other FCV strains. Previous work has demonstrated the presence of hypervariable amino acid sequences in the capsid protein of FCV (designated regions C and E) that were postulated to constitute the major antigenic determinants of the virus. To examine the inv...